西南牡蒿叶绿体基因组特征及系统发育分析

o explore the structural features of Artemisia parviflora chloroplast genome and itssystematic position. We employed high-throughput sequencing technology for genome sequencingand analyzed by bioinformatics tools. The results were as follows: (1) The chloroplast genome ofA. parviflora was 151 047 bp, with a typical circular double-stranded tetrad structure, and the GCcontent was 37.5%. (2) Total 115 unique genes were annotated, including 81 protein-coding genes,four rRNA genes, and 30 tRNA genes. (3) Sixty-eight simple sequence repeats (SSRs) and 37 longrepeat sequences were detected. (4) The codon usage bias was weak in the A. parviflorachloroplast genome, and natural selection mainly contributed to the codon usage bias.High-frequency codons tend to ended with A/U. (5) There was no obvious expansion orcontraction of the inverted repeat (IR) regions. Five high variation regions (trnHpsbA,rpl16rps3, ycf15trnL-UAG, ndhA, and ycf1) were identified which could be used as potentialmolecular markers for identifying Subgen. Dracunculus species. (6) Phylogenetic analysisrevealed the systematic position of A. parviflora within Subgen. Dracunculus and elucidated thephylogenetic relationships among the various subgenera of Artemisia. This study lays thefoundation for future molecular marker development and phylogenetic research of Artemisiaspecies.