水稻条纹病毒胁迫下的水稻全基因组表达谱

Rice stripe disease caused by Rice stripe virus (RSV) resulted in great reduction of rice production in China. To investigate the effects of RSV on rice gene expression, Affymetrix rice whole genome arrays were used to compare the transcriptional profiles of mock-innoculated and RSV-innoculated WuYun3 7 days after stripe symptom appeared. 3517 differently expressed transcripts were found in total, including 2002 up-regulated transcripts and 1515 down-regulated ones in response to RSV infection. According to TIGR annotation (http://www.tigr.org/tdb/e2k1/osa1/) and MIPS function catalogue (http://mips.gsf.de/projects/funcat), these differently expressed genes were classified into 15 functional groups and most of them related to plant defence, signal transduction or metabolism of protein or carbohydrate. Some transcription factors were also regulated significantly by RSV infection. Pathway analysis showed that gene expression of enzymes involved in pentose phosphate pathway, flavonoid pathway and brassinoliele synthesis pathway were notably induced, while expression of enzymes participated in gibberellin synthesis pathway were repressed.