一个假定RhoGEF可能调控稻瘟病菌营养生长和产孢
Putative RhoGEF May Involve in Regulation of Vegetative Growth and Conidiation in Magnaporthe oryzae
鸟苷酸交换因子（GEF) 促使小G蛋白从GDP结合态转换为GTP结合态，而激活G蛋白调控的信号途径，是一类重要的G蛋白活性调控因子。根据基因组注释结果，稻瘟病菌基因位点Mgg_11178.6编码一个假定的Rho族小G蛋白鸟苷酸交换因子（MoRGF1）。系统进化分析表明，MoRGF1与其它真菌同源的RhoGEF有较为紧密的亲缘关系。进一步运用同源重组的方法，获得了该基因敲除的突变体。与对照菌株比较，突变体表现为生长速度减慢，产孢量明显减少，但是致病力没有变化。说明MoRGF1可能参与稻瘟病菌营养生长与产孢过程的调控。
Guanine nucleotide exchange factor, an important G protein regulator, activates the G protein regulated signal pathways by switching on G protein from GDP-bound to GTP-bound state. Based on the genome annotation, the gene locus Mgg_11178.6 encodes a guanine nucleotide exchange factor for Rho GTPases (designated as MoRGF1) in Magnaporthe oryzae. Phylogenetic analysis showed that MoRGF1 shared high identiy with its homologs from other filamentous fungi. Two MoRGF1 deletion mutants, obtained by homologous recombination, displayed slower growth and obviously reduced conidiation, although with no change in pathogenicity when compared with their wild type. Our results suggested the putative RhoGEF may involve in regulation of growth and conidiation in M. oryzae.
林岐、王宗华、石振华、鲁国东、张冬梅
分子生物学微生物学遗传学
稻瘟病菌RhoGEF同源重组基因敲除
Magnaporthe oryzaeRhoGEFhomologous recombinationgene knock-ou
林岐,王宗华,石振华,鲁国东,张冬梅.一个假定RhoGEF可能调控稻瘟病菌营养生长和产孢[EB/OL].(2009-04-01)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200904-53.点此复制
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