香烟烟雾对人支气管上皮细胞FOXO3基因的组蛋白乙酰化及转录与表达的影响

his study aim to investigate the change of histone acetylation and its effect on the expression of FOXO3 gene in the malignant human bronchial epithelial cells (BEAS-2B) induced by cigarette smoke. BEAS-2B cells were maintained in cigarette smoke for long-term exposure; Cell apoptosis was investigated by flow cytometry, and caspase3/7 activity was analyzed; the changes in histone acetylation of malignant cells was analyzed by high-throughput ChIP-Seq detection , the expression level of mRNA and protein of FOXO3 were detected by Realtime-PCR and Western blotting. Results: The caspase3/7 cell activity and apoptosis rate decreased graEffect of Cigarette Smoke on Histone Acetylation, Transcription and Expression of FOXO3 in BEAS-2B Celldually with the increase of tobacco smoke exposure; 155 and 892 genes were determine as high H3K9ac and low H3K9ac in their promoter respectively. Expression of FOXO3 rose up after TSA-treatment. The expression of genes related to apoptosis reduced. Conclusion: low-H3K9ac leads to the decrease of FOXO3 gene expression, which plays an important role in apoptosis escape process of BEAS-2B cell malignant transformation induced by cigarette smoke.