Host-microbe interactions in the chemosynthetic Riftia pachyptila symbiosis
Host-microbe interactions in the chemosynthetic Riftia pachyptila symbiosis
Abstract The deep-sea tubeworm Riftia pachyptila lacks a digestive system, but completely relies on bacterial endosymbionts for nutrition. Although the symbiont has been studied in detail on the molecular level, such analyses were unavailable for the animal host, because sequence information was lacking. To identify host-symbiont interaction mechanisms, we therefore sequenced the Riftia transcriptome, which enabled comparative metaproteomic analyses of symbiont-containing versus symbiont-free tissues, both under energy-rich and energy-limited conditions. We demonstrate that metabolic interactions include nutrient allocation from symbiont to host by symbiont digestion, and substrate transfer to the symbiont by abundant host proteins. Our analysis further suggests that Riftia maintains its symbiont by protecting the bacteria from oxidative damage, while also exerting symbiont population control. Eukaryote-like symbiont proteins might facilitate intracellular symbiont persistence. Energy limitation apparently leads to reduced symbiont biomass and increased symbiont digestion. Our study provides unprecedented insights into host-microbe interactions that shape this highly efficient symbiosis.
Hinzke Tjorven、Kleiner Manuel、Breusing Corinna、Schl¨1ter Rabea、Rosenstiel Philip、Schweder Thomas、Markert Stephanie、Reusch Thorsten B. H.、Felbeck Horst、H?sler Robert、Sievert Stefan M.
Institute of Marine Biotechnology e. V.||Institute of Pharmacy, Department of Pharmaceutical Biotechnology, University of Greifswald||Energy Bioengineering Group, University of CalgaryEnergy Bioengineering Group, University of Calgary||Department of Plant & Microbial Biology, North Carolina State UniversityMonterey Bay Aquarium Research InstituteImaging Center of the Department of Biology, University of GreifswaldInstitute of Clinical Molecular Biology (IKMB), Kiel UniversityInstitute of Marine Biotechnology e. V.||Institute of Pharmacy, Department of Pharmaceutical Biotechnology, University of GreifswaldInstitute of Marine Biotechnology e. V.||Institute of Pharmacy, Department of Pharmaceutical Biotechnology, University of GreifswaldMarine Evolutionary Ecology, GEOMAR Helmholtz Centre for Ocean Research KielScripps Institution of Oceanography, University of California San DiegoInstitute of Clinical Molecular Biology (IKMB), Kiel UniversityBiology Department, Woods Hole Oceanographic Institution
分子生物学微生物学动物学
Metaproteomicsholobiontmutualismmutualistic associationdeep seahydrothermal vents
Hinzke Tjorven,Kleiner Manuel,Breusing Corinna,Schl¨1ter Rabea,Rosenstiel Philip,Schweder Thomas,Markert Stephanie,Reusch Thorsten B. H.,Felbeck Horst,H?sler Robert,Sievert Stefan M..Host-microbe interactions in the chemosynthetic Riftia pachyptila symbiosis[EB/OL].(2025-03-28)[2025-06-23].https://www.biorxiv.org/content/10.1101/651323.点此复制
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