大鼠体内恒释阿霉素肾病综合征模型的制备及真武汤对其影响

Objective: To establish adriamycin-caused rat model of nephritic syndrome (NS)by constant release technology using osmotic pump, and carrying out the theraputic intervention to evaluate the efficacy of Zhenwu decoction on NS.Methods:Osmotic pump filled with adriamycin(14.5mgokg-1)was implanted into rats enterocoelia, then induced the NS on rats by constant release of adriamycin with osmotic pump,and after intervention of Zhenwu decoction, the urine protein，blood pressure ，serum lipid parameters,plasmaprotein ,renal function indicators,andIgGexpressioninglomerulus were detected, which was to assess the establishment of model of NS rat and the efficacy of Zhenwu decoction on it.Results: The survival rate of rats withadriamycin-caused NS using osmotic pump was improved significantly,no rats died during experiment.The NS occurred in the rats of model group at second week after giving driamycin,and compared with control group,the urine protein on 24h and blood pressure were increasedsignificantlyafter model establishment,and blood lipid,as well as markedly decreased serum protein and renal hypofunction were detected(P<0.01);Compared with model group,the mental state,activity, food intake and hair colour of rats were observably improved after administrating lower and higher doses of Zhenwu decoction(24.0,12gokg-1od-1),and also both doses were capable of significantly decreasing urine protein and blood pressure (P<0.01),recovering rats serum protein level (P<0.05),improving BUN and Scr levels, decreasing the content of TC andTG(P<0.01),and the notably decreased IgG deposition in rats mesangialarea.Conclusion: The stable model of rat with NS could be successfully established using constant release function of osmotic pump and after intervention with Zhenwudecoction,it showed theefficacy on improving NS from the aspects of decreasing the urine protein,increasingplasma protein level, relieving hypercoagulablestate,the decline in contents of BUN and Scr and restoring glomerular structure.