中国对虾谷胱肝肽过氧化酶及转硫酶
A selenium-dependent glutathione peroxidase (Se-GPx) and two glutathione S-transferases (GSTs) from Fenneropenaeus chinensis, Chinese shrimp
谷胱肝肽过氧化酶及转硫酶在抗氧化防御中起到关键的作用,我们从中国对虾中克隆到了两个不同亚型的转硫酶基因和一个硒半胱氨酸谷胱肝肽过氧化酶.这三个基因可以应答弧菌的刺激.另外,我们还检测了对虾体内的这两个基因受到弧菌刺激不同时间的总的酶活变化.总之,这三个基因在对虾的抗菌免疫防御中起到关键作用.
生物化学分子生物学动物学
谷胱肝肽过氧化酶转硫酶中国对虾
王金星,任乾,孙荣嵘,赵小凡.中国对虾谷胱肝肽过氧化酶及转硫酶[EB/OL].(2009-01-15)[2025-09-21].http://www.paper.edu.cn/releasepaper/content/200901-670.点此复制
Glutathione S-transferases (GSTs) and glutathione peroxidases (GPx) are essential components of cellular detoxification systems that defend cells against reactive oxygen species (ROS). Two GST genes have recently been cloned from Fenneropenaeus chinensis and BLAST P analysis shows that one GST, designated FcMuGST, is similar to members of MuGST while the other has similarities to ThetaGST (FcThetaGST). A selenium-dependent glutathione peroxidase (Se-GPx) has also been cloned from F. chinensis. The alignment of the deduced GST and GPx amino acid sequences with those from other species showed that the residues essential for enzymatic function of these three proteins are highly conserved. Tissue distribution and response to pathogens for the three genes was investigated by RT-PCR analysis, which showed that the transcript of FcMuGST and FcGPx increased in response to Vibrio anguillarum infection, while FcThetaGST showed little change at the transcript level. GPx activity in gill tissues quickly increased at 6 h after V. anguillarum challenge and maintained at a relatively high level from 6 h to 24 h. Total GST activity in hepatopancreas and intestines of the bacterial challenged shrimp was increased at 6 h, and gradually recovered from 12 and 24 h to the normal level. These three genes were all predicted to play an important role in detoxification defense reactions. FcMuGST primarily scavenges excess ROS produced after bacterial infection, while clearance of endogenous hydrophobic electrophile molecules was mainly dependent on activities of FcThetaGST.
antioxidant defenseFenneropenaeus chinensisGlutathione S-transferasesglutathione peroxidases
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