手机辐射对鸡胚心脏发育的影响

o investigate the effects of Mobile Phone Radiation (MP) on microscopic structure, ultrastructure and Chk1 expression of heart development in chick embryo, DNA damages in blood cells. [Methods] The chick embryos were randomly and evenly divided into control group and exposure group. Embryos in exposure group were irradiated by MP (900/1,900 MHz; SAR≈1.07W/kg) 3h per day from day 2 to day 21 of the incubation of the embryo. Light microscope, electron microscope techniques, single cell gel electrophoresis and western blot were used to study the effects.[Results] Compared to the control group, exposure group had no obvious lesion changes in early embryo development, on the 10th and 13th day , the distribution of lipid droplet in exposure group increased significantly. From day 15 to day 21, the MP radiation caused dispersed disruption of myofilament, focal necrosis of the atrioventricular valve, vacuolization and deformation of mitochondrium in exposure group were evidenced. On the 15th day, the tail area in blood cells in chick embryo heart in the exposure group was higher than that in the control group (p<0.05). Chk1 expression was much higher in both control and exposure group and no obvious difference in early and middle embryo development. But on the 21st day Chk1 expression was only in exposure group. [Conclusions] Mobile Phone Radiation may cause microscopic structure and ultrastructure alterations of myocardial cells, and induce DNA damages. The expression of Chk1 was increased significantly and played an important role in the DNA repairing and cell apoptosis process.