毒鼠强中毒大鼠脑GABA及GABAAR-α1表达的实验研究

bstract：Objective To approach the intoxicated mechanisms we investigated the expression of GABA and GABAAR-α1 in rat poisoned by tetramine. Methods sixty healthy Spragwe-Dawley rats were randomly divided into four groups including the control, the sham poisoned, the high-dose poisoned and the low -dose poisoned. And the latter was once more subdivided into nine subgroups according to the sacrificed time of animals at 30min, 3h, 6h, 12h, 1d, 3d, 5d, 7d, 10d after poisoned. Each rat of high-dose poisoned group was given 1.0mg/kg tetramine by lavage and the rat of chronic poisoned group was given 0.1mg/kg tetramine by lavage. The expression of GABA and GABAAR-α1 were studied by immunohistochemistry and the results were assessed by image analysis system. Results ①Moderate and ubiquitous but definite expression of GABA and GABAAR-α1 was seen in the brain of control and sham poisoned groups. ②The expression of GABA and GABAAR-α1 decreased in the brain of high-dose poisoned group. ③The expression of GABA decreased at first in the brain of low-dose poisoned group, and was lowest level in 6 hours after poisoned, then it enhanced and reached at peak in 5~7 days after poisoned, it was remain above the control till 10 days. ④The expression of GABAAR-α1 in the brain of low-dose poisoned group also decreased at first and was lowest level in 6~12 hours after poisoned, then it enhanced and reached the level of the control group in 7~10 days after poisoned.