农杆菌介导大豆转化OsAPX2基因研究

In this study, putative OsAPX2-over-expression soybean lines were obtained by Agrobacterium-mediated cotyledonary-node method. Subsequently, PCR and DNA sequencing were performed to detect the transgenic lines. The results of PCR amplification and DNA-sequencing in T0 and T1 plants showed that 1268 bp bands were clearly electrophoresed on the gel, and the sequence of the bands were consistent with the original sequence of OsAPX2, which demonstrated that OsAPX2 were successfully integrated into the genome of soybean. The results of RT-PCR showed that OsAPX2 were stably expressed in the transgenic soybean plants, and the transcript accumulations of L33 and L34 transgenic lines were significantly increased than the others. Drought resistance determination showed that these two transgenic lines (L33, L34) had stronger capability to tolerate drought stress, compared with wild-type, which suggested that over-expression of OsAPX2 might enhance soybean drought-resistance.