艾灸预处理对急性胃溃疡大鼠胃黏膜增殖修复的影响

IM: Observation the effects on proliferation and restoring of gastric mucosa in rats with acute gastric ulcer by pre-moxibustion, analyze whether this effect is associated to Gastric Mucosa Blood Flux (GMBF), the content of Prostaglandin E2 (PGE2) and Transforming Growth Factor α(TGF-α) , reveal the mechanism of moxibution at “Zusanli” and “Liangmen” to accelerate the repair of gastric mucosal lesion and protecting gastric mucosa . METHODS: Sixty healthy SD rats were randomly assigned into 4 groups ,named as binding controlgroup(A) ,modelgroup(B). moxibustion at “Zusanli” and “Liangmen” (B)and moxibustion at non-acupoints control groups(D).the animal model of acute gastric ulcer was established by water immersion and restraint stress, the rats in groupA, B, and D served as the restraint, model, and non-accupoint controls, while those in group C received moxibustion at Zusanli and Liangmen.Biological signal analyzer was applied to measure gastric mucosal blood flow (GMBF). The level of PGE2 and TGF-α was detected by radioimmunoassay. Immunohistochemical method was used to detect the Proliferating Cell Nuclear anti-gen (PCNA) in all the groups, RESULTS: Pre-moxibustion at Zusanli and Liangmen point significantly decreased the gastric mucosal injury index (UI), increased the gastric mucosal blood flow (GMBF) ,the content of PGE2 and TGF-αin gastric mucosa; enhanced expression of PCNA (P<0.05或P<0.01). CONCLUSIONS: Pre-moxibustion at “Zusanli” and “Liangmen” could accelerate to restore the gastric mucosa injury by water immersion restraint, the mechanism maybe concern with the increase of content of PGE2 and TGF-αin gastric mucosa ,improving GMBF and accelerate gastric mucosal cell proliferation.