原位杂交技术检测miRNA-34c在小鼠睾丸和精原干细胞的表达
miRNA-34c expression in mouse testis and spermatogonial stem cells(SSCs) identification by FISH
目的：研究miR-34c在小鼠睾丸组织和小鼠精原干细胞中的表达规律。方法：应用FISH (Fluorescence in situ hybridization)技术完成在睾丸组织切片和精原干细胞的miR-34c示踪。结果：实验结果表明miR-34c在2月龄小鼠睾丸中表达量较高；在转染入miR-34c 模拟物组miR-34c 表达量较其他组高。结论：使用FISH方法探索miRNA 表达规律较稳定，且重复率高。
Objective: To explore the expression of miR-34c in mouse testis tissues and mouse spermatogonial stem cells. Methods: To illustrate the miR-34c expression, microRNA FISH (Fluorescence in situ hybridization) was used. Results: miR-34c expressed higher in 2m mouse testis tissue slices and the group of miR-34c mimics transfected SSCs. Conclusion: To measure miRNA expression, FISH is a stable and repeatable method.
于萌、华进联、刘维帅
分子生物学细胞生物学遗传学
发育生物学FISHSSCs睾丸小鼠
evelopmental biologyFISHspermatogonial stem cellstestismouse
于萌,华进联,刘维帅.原位杂交技术检测miRNA-34c在小鼠睾丸和精原干细胞的表达[EB/OL].(2013-05-29)[2025-05-24].http://www.paper.edu.cn/releasepaper/content/201305-454.点此复制
评论