阿托伐他汀对自发性高血压大鼠血管平滑肌细胞凋亡及p27蛋白表达的影响

IM The aim of this study was to evaluate the effects of atorvastatin on aortic smooth muscle cells (ASMC) apoptosis and p27 expression in spontaneously hypertensive rats (SHR). METHODS 12 eight-week-old SHR were randomized into atorvastatin group (ATV, n=6) and SHR group (n=6). 6 age-matched normotensive Wistar-Kyoto rats (WKY) were as controls. 50mg.kg-1.d-1 of atorvastatin was administered to ATV by gavage for 10 weeks. Serum cholesterol and triglycerides were measured, and systolic blood pressure of caudal artery was examined. ASMC apoptosis rate was detected by TUNEL technique, and positive expression rate of p27 in ASMC was analyzed. RESULTS After 10 weeks, systolic blood pressure in ATV was significantly lower than that in SHR (134.17±3.60 vs. 173.33±3.78 mmHg, P<0.01). Compared with SHR, serum cholesterol and triglycerides were significantly lower (P<0.01, P<0.01) in ATV. ASMC apoptosis rate was higher in ATV than that in SHR (16.94±3.08 vs. 9.01±2.36%, P<0.01). Compared with WKY, positive expression rate of p27 in ASMC from ATV was higher (24.25±4.41 vs. 33.02±5.01%, P<0.05), whereas that was lower in SHR (24.25±4.41 vs. 16.08±7.09%, P<0.01). CONCLUSION Atorvastatin may up-regulate p27 expression in ASMC and facilitate ASMC apoptosis which may effectively reduce blood pressure in SHR.