Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability
Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability
Abstract Water stress is a major environmental factor affecting Citrus spp. and Rangpur lime is a drought-tolerant rootstock used to enhance orange yield in rainfed orchards. Here, we combined morpho-physiological analyses with metabolic profiling of roots and leaves of Valencia orange scions grafted onto Rangpur lime, Swingle citrumelo or Sunki mandarin rootstocks under water deficit. Our aim was to present a comprehensive spatio-temporal evaluation of citrus responses to drought and highlight the metabolic adjustments associated with drought tolerance induced by Rangpur lime. Plant responses were evaluated during the initial phase of reduction in water availability, when water deficit was maximum and also after rehydration. Fifty-eight primary metabolites were modulated by water deficit, mainly amino acids, organic acids and sugars. Metabolic changes indicated adjustments related to osmotic, energetic and redox processes under low water availability, which were dependent on rootstock and varied between roots and leaves and along the experimental period. Rangpur lime prioritized root growth in the initial phase of water deficit, which was linked to less sugar accumulation, changes in nucleotide metabolism, downregulation in Shikimic acid pathway and accumulation of arginine. After rehydration, the resume of shoot growth was associated with high accumulation of arginine and asparagine. The better performance of Rangpur lime seems to be associated with its high sensitivity of roots to changes in water availability and possible signaling compounds have been suggested.
Machado Eduardo C.、Ribeiro Rafael V.、Miranda Marcela T.、Domingues-Jr Adilson P.、Aricetti Juliana A.、Silva Simone F.、Caldana Camila、Cunha Camila P.
Agronomic Institute (IAC), Center for Research and Development of Agricultural Biosystems and Post-Harvest, Laboratory of Plant Physiology ?°Coaracy M. Franco?±University of Campinas (UNICAMP), Institute of Biology, Department of Plant Biology, Laboratory of Crop PhysiologyUniversity of Campinas (UNICAMP), Institute of Biology, Department of Plant Biology, Laboratory of Crop Physiology||Agronomic Institute (IAC), Center for Research and Development of Agricultural Biosystems and Post-Harvest, Laboratory of Plant Physiology ?°Coaracy M. Franco?±University of Campinas (UNICAMP), Institute of Biology, Department of Plant Biology, Laboratory of Crop PhysiologyBrazilian Biorenewables National Laboratory (LNBR), National Center for Research in Energy and Materials (CNPEM)University of Campinas (UNICAMP), Institute of Biology, Department of Plant Biology, Laboratory of Crop PhysiologyMax Planck Institute for Molecular Plant PhysiologyUniversity of Campinas (UNICAMP), Institute of Biology, Department of Plant Biology, Laboratory of Crop Physiology
农业科学研究植物学生物化学
abiotic stressmetabolomeroot systemprimary metabolitesRangpur lime
Machado Eduardo C.,Ribeiro Rafael V.,Miranda Marcela T.,Domingues-Jr Adilson P.,Aricetti Juliana A.,Silva Simone F.,Caldana Camila,Cunha Camila P..Metabolic profiling of drought tolerance: revealing how citrus rootstocks modulate plant metabolism under varying water availability[EB/OL].(2025-03-28)[2025-05-05].https://www.biorxiv.org/content/10.1101/2022.07.09.499439.点此复制
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