视网膜母细胞瘤和正常视网膜组织中VEGF-C表达和淋巴管计数的研究

Objective to investigate the VEGF-C expression and lymph vessel counts in retinoblastoma (RB) and normal retina tissue, to study their interrelationship and clinicopathological significance. Method Envision immunohistochemistry stainings of VEGF-C and D2-40 are performed in 30 retinoblastoma and 15 normal retina tissues, which is routinely paraffin imbedded. Result the positive rate of VEGF-C expression and lymph vessel counts is higher in RB tissue than normal control (P<0.01); Samples from the clinical stage I, differentiated type, uninvolved optic nerve and life span ≥2 year cases have higher expression of EMMPRIN、MMP1、MMP9 than those from clinical stage III, undifferentiated type, involved optic nerve and life span <2 year cases(P<0.05 or P<0.01). lymph vessel counts in VEGF-C positive samples are much higher than negative ones. Conclusion VEGF-C expression and lymph vessel counts maybe a useful marker of RB progression, biological behavior and prognosis; VEGF-C might have promotive effect on lymphangiogenesis in RB tissue.