One Health or Three? Transmission modelling of Klebsiella isolates reveals ecological barriers to transmission between humans, animals and the environment
One Health or Three? Transmission modelling of Klebsiella isolates reveals ecological barriers to transmission between humans, animals and the environment
Abstract The Klebsiella group is highly diverse both genetically and ecologically, being commonly recovered from humans, livestock, plants, soil, water, and wild animals. Many species are opportunistic pathogens, and can harbour diverse classes of antimicrobial resistance (AMR) genes. K. pneumoniae is responsible for a high public-health burden, due in part to the rapid spread of health-care associated clones that are non-susceptible to carbapenems. Klebsiella thus represents a highly pertinent taxon for assessing the risk to public health posed by animal and environmental reservoirs. Here we report an analysis of 6548 samples and 3,482 genome sequences representing 15 Klebsiella species sampled over a 15-month period from a wide range of clinical, community, animal and environmental settings in and around the city of Pavia, in the northern Italian region of Lombardy. Despite carbapenem-resistant clones circulating at a high frequency in the hospitals, we find no genotypic or phenotypic evidence for non-susceptibility to carbapenems outside of the clinical environment. The non-random distribution of species and strains across sources point to ecological barriers that are likely to limit AMR transmission. Although we find evidence for occasional transmission between settings, hierarchical modelling and intervention analysis suggests that direct transmission from the multiple non-human (animal and environmental) sources included in our sample accounts for less than 1% of hospital disease, with the vast majority of clinical cases originating from other humans.
Mitchell Sonia、David Sophia、Merla Cristina、Marone Piero、Brisse Sylvain、Matthews Louise、Fernandez Juan Sebastian Lopez、Feil Edward J.、Ferrari Carolina、Corbella Marta、Couto Natacha、Passet Virginie、Thorpe Harry、Corander Jukka、Reeve Richard、Sassera Davide、Rodrigues Carla、Booton Ross、Gibbon Marjorie J.、Comandatore Francesco、Kallonen Teemu
Boyd Orr Centre for Population and Ecosystem Health, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of GlasgowCentre for Genomic Pathogen Surveillance, Wellcome Sanger InstituteMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San MatteoMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San MatteoInstitut Pasteur, Biodiversity and Epidemiology of Bacterial PathogensBoyd Orr Centre for Population and Ecosystem Health, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of GlasgowInstitut Pasteur, Biodiversity and Epidemiology of Bacterial PathogensThe Milner Centre for Evolution, Department of Biology and Biochemistry, University of BathMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San MatteoMicrobiology and Virology Unit, Fondazione IRCCS Policlinico San MatteoThe Milner Centre for Evolution, Department of Biology and Biochemistry, University of BathInstitut Pasteur, Biodiversity and Epidemiology of Bacterial PathogensDepartment of Biostatistics, University of OsloDepartment of Biostatistics, University of Oslo||Centre for Genomic Pathogen Surveillance, Wellcome Sanger InstituteBoyd Orr Centre for Population and Ecosystem Health, Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences, University of GlasgowDepartment of Biology and Biotechnology, University of PaviaInstitut Pasteur, Biodiversity and Epidemiology of Bacterial PathogensBristol Veterinary School, University of BristolThe Milner Centre for Evolution, Department of Biology and Biochemistry, University of BathRomeo ed Enrica Invernizzi Pediatric Research Center, Department of Biomedical and Clinical Sciences Luigi Sacco, Universit¨¤ di MilanoDepartment of Clinical Microbiology, Turku University Hospital
医学研究方法环境科学理论微生物学
KlebsiellaecologyWhole-Genome sequencingepidemiologyAMRtransmissionOne-Health
Mitchell Sonia,David Sophia,Merla Cristina,Marone Piero,Brisse Sylvain,Matthews Louise,Fernandez Juan Sebastian Lopez,Feil Edward J.,Ferrari Carolina,Corbella Marta,Couto Natacha,Passet Virginie,Thorpe Harry,Corander Jukka,Reeve Richard,Sassera Davide,Rodrigues Carla,Booton Ross,Gibbon Marjorie J.,Comandatore Francesco,Kallonen Teemu.One Health or Three? Transmission modelling of Klebsiella isolates reveals ecological barriers to transmission between humans, animals and the environment[EB/OL].(2025-03-28)[2025-06-13].https://www.biorxiv.org/content/10.1101/2021.08.05.455249.点此复制
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