小鼠CD19胞膜外区基因的克隆与原核表达
loning and prokaryotic expression of the extracellular domain of mouse CD19 gene
为了解小鼠CD19胞膜外区（the extracellular domain of mouse CD19,MECD19）的结构与功能，作者应用反转录-聚合酶链式反应技术（RT-PCR），通过一对自行设计的引物，从小鼠脾脏RNA中扩增出一条特异性基因片段，大小约为918bp，该片段经酶切和测序鉴定为小鼠CD19胞膜外区基因，将其插入原核表达载体pGEX-4T-1，并导入大肠杆菌BL21细胞中，经IPTG诱导培养，获得分子量约为59000的蛋白带。该结果表明所克隆的小鼠CD19胞膜外区基因在大肠杆菌原核表达系统中成功地得到表达。
im to lay the foundation for investigating the structure-function relationship of the extracellular domain gene of mouse CD19(MECD19), a specific DNA fragment with a length of 918bp was cloned from mouse spleen lymphocytes by reverse transcription-polymerase chain reaction (RT-PCR) with a pair of designed primers. After an endonuclease and sequencing, this fragment was identified as the extracellular domain gene of mouse CD19, then it was inserted into PGEX-4T-1, a prokaryotic expression plasmid, and expressed in Escherichia coli BL21, which were further induced by IPTG and cultured. From the culture a fusion protein was obtained with about 59000 of molecular weight in SDS-PAGE. The extracellular domain gene of mouse CD19 in prokaryotic expression system was successfully expressed.
李林、王静、余为一
分子生物学生物工程学
小鼠19克隆原核表达
mouse CD19 cloning prokaryotic expression
李林,王静,余为一.小鼠CD19胞膜外区基因的克隆与原核表达[EB/OL].(2007-04-25)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200704-632.点此复制
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