多功能纳米材料CDPF/CDP运载AEG-1 siRNA抑制骨肉瘤细胞增殖和侵袭能力的研究
Inhibition of proliferation and invasion of human osteosarcoma cell line by multi functional nano material CDPF/CDP carrying siRNA AEG-1
目的 探讨多功能纳米材料CDPF和CDP运载AEG-1 siRNA进入骨肉瘤细胞能力以及其对细胞增殖、侵袭能力的影响。方法 通过体外细胞瞬时转染实验将CDPF/AEG-1 siRNA或CDP/AEG-1 siRNA运载入143B细胞，用Western-blot检测AEG-1 蛋白水平的变化，分别用MTT法和侵袭实验检测转染复合物对143B细胞增殖能力和侵袭能力的影响。结果 CDPF和CDP成功的将AEG-1 siRNA运载至143B细胞，与对照组比较，AEG-1蛋白水平显著降低，下调AEG-明显抑制143B细胞的增殖和侵袭能力，且CDPF比CDP具有更好的优势。结论 CDP可作为有效的新型基因转染载体，叶酸的接入可提高其效率。
Objective：To explore the ability of multi-functional nano carriers CDP and CDPF to load AEG-1 siRNA into osteosarcoma cells and the effect of its on cell proliferation and invasion ability. Methods: CDPF/AEG-1 siRNA or CDP/AEG-1 siRNA was loaded into 143B cells by transient transfection assay in vitro. Expression of AEG-1 protein was detected by Western blot. The cell proliferation was detected by MTT method and the invasion ability was analyzed by invasion assay. Results: CDP and CDPF successfully carried AEG-1 siRNA into 143B cells. Compared with control groups, the expression of AEG-1 protein was evidently decreased. Down-regulating the expression of AEG-1 decreased the cell proliferation and invasion ability. Conclusion: CDP can be used as a new gene transfer vector, and folic acid can improve its efficiency.
庞家栋、张黎明、王芬
肿瘤学基础医学生物科学研究方法、生物科学研究技术
纳米颗粒壳聚糖树状聚赖氨酸EG-1 siRNA骨肉瘤
nanoparticlechitosandendritic poly(L-lysine)EG-1 siRNAosteosarcoma
庞家栋,张黎明,王芬.多功能纳米材料CDPF/CDP运载AEG-1 siRNA抑制骨肉瘤细胞增殖和侵袭能力的研究[EB/OL].(2016-11-11)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201611-169.点此复制
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