Jagged-1对Th17细胞分化影响的初探

soluble Jagged-1/Fc chimera protein (Jagged-a/Fc) was directly used to induce in vitro differentiation of murine lymph node cells to explore primarily the effect of activation of Notch signaling on the differentiation of Th17 cells. First, the lymph node cells were treated with different concentrations of Jagged-1 at different time. Then, interleukin 17(IL-17), interleukin 4(IL-4), interleukin 6(IL-6) and interleukin 10 (IL-10) in the culture supernatant from the treated cells were detected by ELISA. And expression of NICD, Hes-1 and Deltex-1 was determined through Western Blot, respectively. Flow cytometry was applied to analyze the effect of Jagged-1 on the intracellular level of IL-17 in CD4+ T cells. The results showed that, as the concentration of Jagged-1 was increased, expression of NICD, Hes-1 and Deltex-1 was obviously up-regulated, the level of IL-10 also was increased, whereas expression of IL-17 was decreased, which could be offset by DAPT or anti-Jagged-1 antibody. The results indicate that Jagged-1 could up-regulate Notch signaling, suppressing the differentiation of peripheral T- lymphocytes into Th17 cells.