胃癌中maspin和突变型p53蛋白的表达及临床病理学意义

Objective: To detect the expressions of maspin and mutant p53 protein, and to analyze the correlation of maspin protein expression with pathological typing, staging of gastric carcinoma, lymphnode metastasis and the expression of mutant p53 protein so as to investigate its biological significance and related molecular pathological mechanism in the carcinogenesis and progress of gastric carcinoma. Methods: PV9000 immunohistochemical method was used to detect the expression of maspin and p53 in156 cases of tumor specimens and 105 cases of matched normal gastric mucosa and , 106 intestinal metaplasia (IM), 6 dysplasia (Dys). Results: There were significant differences of maspin expression among the gastric cancer tissues (51.3%), intestinal metaplasia(95.3), and normal gastric mucosa. There were no statistical difference of the positive rates of maspin protein expression in intestinal type and diffuse type gastric carcinoma, different stages of gastric carcinoma, with and without lymph node metastasis. The rank correlation detection revealed that there was no rank correlation between expressions of maspin and p53 protein in gastric carcinoma tissues(P>0.05), while spearman rank correlation coefficient was -0.06 in gastric carcinoma of intestinal type, indicating that there was a negative correlation tendency. Conclusions：Inactivation of maspin expression may play an important role during the progress of intestinal metaplasia transforming to the gastric carcinoma. In gastric carcinoma, the expression of maspin expression had on evident rank correlation with that of mutant p53 protein,while in intestinal-type gastric carcinoma, negative correlation tendency occurs between them. Further studies are needed on the certain role and molecular biological mechanism of maspin in the carcinogenesis and metastasis of gastric carcinoma.