长链非编码RNA在新生大鼠缺氧缺血脑组织中的表达

Objective To investigate the expression of long non-coding RNA (lncRNA) in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods Microarray was used to detect the expression of lncRNA and mRNA in hypoxic-ischemic group and sham control brain. The expressed lncRNA were detected by real-time PCR. The differentially expressed mRNA was analyzed by gene ontology (GO), pathway and coding-noncoding RNA co-expression (CNC) network analysis. Results Compared to sham control, 322 lncRNAs and 375 mRNAs were significantly altered in neonatal brain following hypoxic-ischemic injury. GO analysis showed that the most enriched biological process associated with the upregulated mRNA was response to wounding, whereas the biological process most enriched among the downregulated mRNA was somatic stem cell division. Pathway analysis indicated that upregulated mRNA primarily corresponded to cytokine-cytokine receptor interaction pathway and that downregulated mRNA mainly correlated to axon guidance pathway. CNC network analysis demonstrated that 177 lncRNAs were correlated to the expression of mRNA involved in inflammation and cell death. Conclusion HI injury significantly influences cerebral lncRNA and mRNA expression profiles in the neonatal rat brains. Deregulated lncRNAs might contribute to the pathogenesis of HIBD via interacting with mRNA.