鲨肝肽基因在家蚕中的表达及其口服吸收的初步研究

PSL (active peptide from shark liver) gene was obtained from the chiloscyllium plagiosum regenerated liver cDNA library. It was reported that APSL could repair damage in islet, and promote recovery of islet function. The APSL gene was expressed by E. coli prokaryotic expression system, and polyclonal antibody was obtained by purified recombinant protein after immunizing New Zealand rabbits. Then the recombinant virus rBv-APSL was constructed by using Bac-to-Bac baculovirus expression system. The recombinant virus was amplified and P4 recombinant virus was got after passaging through three generations. P4 virus infected silkworm larvae, and the expressed rAPSL was detected by Western blotting. Prepare two rAPSL oral formulations to carry out preliminary study on oral administration. The presence of rAPSL in serum of ICR mouse orally administered rAPSL was detected by ELISA. The ELISA results indicated rAPSL could be absorbed into ICR mouse after oral administration of the two formulations, making foundation for study on oral formulations of rAPSL. From the point of restoration of islet function, the medical workers are in eager expectation of carrying out the treatment of diabetes, and this study may make a positive contribution.