茶树花粉总RNA提取方法研究

otal RNA extracted from Camellia sinensis pollen with four methods of CTAB-LiCl, CTAB+adsorption column RNA extraction method, Trizol kit and RNA kit for plant rich in polysaccharides and polyphenol was compared in this study. The results showed that higher yield RNA with good quality was isolated with RNA kits. The electrophoresis assay showed that bands of 28S and 18S were all clear. Further experiments showed that the total RNA could be well specific amplifying of gene fragments after reverse transcription. The results confirmed that the total RNA could be well used for future gene cloning and expression studies.