模式植物番茄Micro Tom的微繁殖和早熟条件优化

Micro Tom is a novel breed model organism of Lycopersicon esculentum Mill. that has the traits of short life cycle, dwarf and miniature. Owing to these characters , the utilization of Micro Tom can decrease the period of scientific research greatly and save space for breeding, so its application is more and more broad. In this paper, we successfully induced the regeneration seedlings of Micro Tom to bear fruits in advance by controlling and optimizing several external such as plant height of the regeneration seedlings, culture medium,the photoperiod and the temperatures. The cotyledon explant was the optimization explant to get regenerated shoots.The condition for inducing regeneerated shoots is MS medium supplemented with 0.2mg/L IAA+3mg/L BA,and treated with low temperature .The condition for inducing prematurity is MS medium supplemented with 0.1 mg/L NAA and 0.5 mg/L IBA,seedlings of 2.0-2.5 cm height,18-20h photoperiod,22-26℃ culturing temperature.All these findings make way for regenerateing Micro Tom plentiful and precocity in in vitro.