JMJD6参与调节肿瘤抑制因子p53的转录活性
JMJD6 regulates the transcriptional activity of p53
目的 确定JMJD6与转录因子p53的相互作用，研究JMJD6对p53转录活性的影响，并通过鉴定JMJD6的赖氨酸羟化酶活性探讨其可能的作用机制。方法 免疫共沉淀技术和GST pull-down实验分析JMJD6和p53的相互作用及其作用区域；荧光素酶双报告基因系统检测JMJD6对p53靶基因启动子活性的影响；体外羟基化实验和质谱鉴定JMJD6的羟化酶活性。结果 JMJD6与p53相互作用，并由p53的C端结构域介导；JMJD6参与激活p53的转录活性；JMJD6具有赖氨酸羟化酶活性，可以催化组蛋白H4发生羟基化。结论 赖氨酸羟化酶JMJD6可以结合肿瘤抑制因子p53并参与调节其转录活性。
Objective To verify the interaction between JMJD6 and p53. To detect the effect of JMJD6 on transcriptional activity of p53. To identify the lysyl-hydroxylase activity of JMJD6. Methods The interaction between JMJD6 and p53 was examined by co-immunoprecipitation and GST pull-down assay. The effect of JMJD6 on p53 target gene promoter activity was detected by dual-luciferase reporter system. The lysyl-hydroxylase activity of JMJD6 was identified by hydroxylation assay in vitro and LC-MS analysis. Result JMJD6 interacts with p53 by p53 C terminal fragment. JMJD6 promotes the transcriptional activity of p53. JMJD6 is a lysyl-hydroxylase catalyzing hydroxylation of histone H4. Conclusion The lysyl-hydroxylase JMJD6 interacts with p53 and regulates its transcriptional activity.
程谟斌、代辉、张业、张艳君
基础医学分子生物学肿瘤学
生物化学与分子生物学p53JMJD6赖氨酸羟基化
Biochemistry and molecular biologyp53JMJD6lysyl-hydroxylation
程谟斌,代辉,张业,张艳君.JMJD6参与调节肿瘤抑制因子p53的转录活性[EB/OL].(2012-10-23)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201210-220.点此复制
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