利用比较基因组学对QPm.cass-4DL进行精细定位

he genome sequences of rice and Brachypodium distachyon were very similar to wheat genome, which is very important to fine mapping some useful genes in wheat. In the present study, we used comparative genomics analysis between wheat, rice and Brachypodium genomic collinearity to confirm that the powdery mildew resistance locus QPm.caas-4DL in wheat chromosome 4DL was orthologous to Brachypodium chromosome 1 and rice chromosome 3. A total of 10 STS (sequence Tagged site) have been developed and only one STS (named as CV6) has been mapped to the linkage map within 0.3 cM from the resistance locus. This molecular marker can be used in wheat breeding program to bread powdery mildew resistant wheat cultivar by marker assistant selection.