体外牙髓干细胞定向分化为成牙本质样细胞的模型研究

o construct odontoblast-like cells’ differentiated models in vitro by inoculated dental pulp cells onto processed dentin discs,evaluate the efficacy of the models . Primary cultures of human dental pulp cells were seeded onto treated dentin discs.For the process of cells’adherence and morphologic change ,samples were collected on day 1，2，4 and 8. Results were analyzed by scanning electron microscope(SEM) and fluorescence microscopy . Samples from the function study were retrieved on day 1,3,6,8 and 10, and evaluated by measuring cell proliferation rate ,alkaline phosphatase (ALP) activity.In the differentiated models, DPSCs can form ambo-layers growth in the earlier period,meanwhile,some cells oriented differentiated into odontoblast-shaped cells. On the dentin discs, the skeleton of microfilaments recombinate(the microfilaments were organized in parallel stress fibre oriented depending on the longitudinal axis of the cytoplasm), obviously step down the DPSCs’ proliferative activity and increase the activity of alkaline phosphatase . The result indicated that the odontoblast-like cells’ differentiated models have the ability to promote the DPSCs differentiating into odontoblasts.The dentin discs can be used to construct odontoblast-like cell’s differentiated models, promoting DPSCs differentiate into odontoblasts.