转座子随机插入鉴定肠炎沙门氏菌生物膜形成相关基因

Biofilm plays an important role in pathogenicity of Salmonella and food poisoning caused by Salmonella. To identify genes associated with Salmonella biofilm formation, 74 strains of Salmonella enterica serovar enteritidis (S. enteritidis), S. pullorum and S, gallinarum isolated from chickens were determined for biofilm using crystal violet staining. Eighty-four percent of these Salmonella strains produced biofilm on the plastic surface and the strain C050041 with well biofilm formation was chosen to construct a mutant library using transposon mutagenesis. The mutants with decreased biofilm formation were sequenced and 15 insertion sites were found. These genes included metE, ompR, rpoS, rfaG, rfaJ, rfaK, rfaP, rfbH, rhlE, spiA, steB, tpx, ybdN and other two genes with unknown function. These findings may help to understand the regulation mechanism of Salmonella biofilm formation and develop an attenuated Salmonella vaccine.