甘蔗纤维素合成酶家族的基因组演化

ccording to the comparative genomics analysis between sorghum and Saccharum species, hybridization probes for eleven cellulose synthase family genes were designed for screening a Saccharum spontaneum BAC library. The results showed that, besides CesA8, ten of orthologous cellulose synthase gene family members in the BAC library were identified and sequenced. The absent orthologous gene, CesA8 were cloned with cDNA template PCR.The analysis of gene annotation and sequence comparison demonstrated that the orthologous gene pairs of sorghum and sugarcane shared high sequence similarity. The phylogenetic genetic analysis were performed for momocotyledon(sugarcane, rice, maize and sorghum) and dicotyledon (Arabidopsis and grape) of cellulose synthase gene family, suggesting that there were at least six ancestor genes for the two classes and SsCesA2 was rapidly evolving compared to the other genes. Based on previous studies for the CesA family in the model plants(Arabidopsis and rice) and phylogenetic genetic classification, SsCesA1, SsCesA6 and SsCesA9 were predicted to play important roles in primary cell wall formation, and SsCesA10, SsCesA11 and SsCesA12 were indispensable for secondary cell wall formation. This study provided the foundation for further investigate the function of CesA family and molecular mechanism of cellulose synthase in sugarcane.