枸杞叶总黄酮对UVB辐射导致的光损伤无毛小鼠皮肤组织MDA、Hyp含量及SOD、GSH-Px、CAT活力的影响

Objective: To study the influence of topical using total flavonoids from Lycium barbarum leaves cream on the skin-photodamaged caused by UVB radiation. Methods: 60 hairless mice were randomly divided into 6 groups, in addition to the control group, with UVB irradiation 2h/day for two weeks. UVB direct exposure to UVB model group. Before the irradiation, the base group smear cream base. And the treatment groups were irradiated to dorsal skin after topical using low, medium and high doses of total flavonoids from Lycium barbarum leaves cream. Determinating minimal erythema dose(MED), histological changes of skin tissue structure, the activities of SOD, GSH-Px, CAT, and the content of MDA and Hyp in skin tissue. Results: After 14 days, MDA content of model group increased, Hyp content decreased, the activities of SOD, GSH-Px, CAT decreased significantly (P<0.01), mice skin tissue histology showing photoaging state and inflammation. Compared with the model group, the MED of Lycium barbarum leaves flavonoids high-dose group was significantly increased (P<0.01), and the SPF=20, the photodamage was midigated, the content of MDA in dorsal skin tissue was significantly reduced, Hyp content was increased, the activities of SOD, GSH-Px and CAT were increased(P<0.01). Conclusion: Topical using total flavonoids from Lycium barbarum leaves can prevent skin damage caused by UVB radiation.