不同剂量TGF-β1对颞下颌关节炎软骨下骨的影响

Objective This study sought to evaluate the effects of intra-articular injection of different dose of TGF-β1 on the subchondral cancellous bone repair in osteoarthritis (OA) of temporomandibular joint (TMJ). Methods Partial disc perforation was performed bilaterally to induce an osteoarthritic joint in 36 animals. 20, 40, or 80 ng TGF-β1 were injected into the right joint, and the contralateral joint received vehicle injection. 4 additional animals were used as normal control. The animals were sacrificed at 6 and 12 weeks after the first injection, respectively, for histology and micro-CT examinations.Micro-CT scanned to quantify three-dimensional microarchitecture of subchondral bone. Results All TGF-β1-treated joints were covered by a layer of well-organized fibrocartilage, and had normal mictoarthitectural properties, whereas the vehicle-treated joint had typical OA-related cartilage degradation and subchondral bone sclerosis.. Conclusion These results suggested that TGF-β1 administration is an effective therapy for intervention of subchondral bone lesions in TMJ OA.