补阳还五汤抑制缺血再灌注肾损伤小鼠TGF-βSmad及PDGFERK通路活化

In order to elucidate the mechanism of Buyang-Huanwu decoction in TGF-β/Smad signaling pathway in AKI-to-CKD mice. We constructed animal model of AKI-to-CKD by renal ischemia-reperfusion injury (IRI). We grouped the mice, assessed renal function on the 30th day after modeling, tested the level of α-SMA and TGF-β/Smad pathways, and detected the changes of Fut8 and VEGF R2 levels and core fucosylation of TGF-βRⅡ. The results show that Buyang-Huanwu decoction also down-regulates the level of Fut8 and core fucosylation of TGF-βRII, up-regulates the level of VEGF R2, inhibits the activation of TGF-β/Smad, and eases the process of IRI. These data indicate that Buyang-Huanwu decoction could inhibit the activation of TGF-β/Smad pathway by inhibiting the Fut8 and then block the pathological process of AKI-to-CKD.