利用PacBio三代测序技术对婴儿配方奶粉中细菌污染的评价

Infant formula is an effective formulated substitute that is designed to mimic the nutritional composition of breast milk for newborn infants. A lot of researches focused on the bacterial contamination of infant formula during the manufacturing process and the influence of sterilization on the quality and safety of product shelf life. The bacterial contamination of 30 infant formula samples (12 of the domestic and 18 of import) was investigated by Pacific Biosciences (PacBio) single molecule, real-time sequencing technology (SMRT) of the full length 16S rRNA gene. The major contaminants were Lactococcus、Streptococcus、Bacillus、Anoxybacillus、Lactobacillus、Solibacillus、Lysinibacillus、Pseudomonas and Acinetobacter. The relative abundances of the detectable bacterial contaminants varied between the domestic and imported infant formula. Higher amounts of Bacillus cereus and Geobacillus stearothermophilus present in the samples may reflect the degree of preheating and the triggering of bacterial spore germination and growth. Our study has demonstrated for the first time the feasibility of using the SMRT sequencing system in detecting the bacterial contamination in dairy infant formula, which would be of interest to the food industry for effective monitoring of microbial food quality and safety during production.