绒山羊70天胎儿皮肤小分子RNA文库构建及miRNA鉴定

miRNA are single-stranded RNA molecules of about 21–23 nucleotides in length. Recently, the miRNAs air found that plays an important role on of the hair follicle development and the maintenance. In order to study the role of the miRNAs during hair follicle development of the cashmere goat, a small RNA library is constructed from the 70-days cashmere goat fetal skin, then the plasmids are extracted from 192 randomly selected clones for sequencing. These sequences are screening and comparing in GenBank and mirBase by Blastn, the result indicated that 32 sequences are found which are highly homologous to other animal miRNAs. These miRNAs classify into 19 different miRNAs, and there are 9 have registered in GenBank, and other 10 are the new cashmere goats miRNAs. miR-199a* and miR-424 have the maximum number of copies, respectively 6 and 3 and indicate they are very active during the hair follicle development of cashmere goat that may have important biological functions. This study is a foundation for research the role of these miRNAs during the hair follicle development of cashmere goat.