Chloroplast activity provides in vitro regeneration capability in contrasting cultivars
Chloroplast activity provides in vitro regeneration capability in contrasting cultivars
Abstract Existence of potent in vitro regeneration system is a prerequisite for efficient genetic transformation and functional genomics of crop plants. We know little about why only some cultivars in crop plants are tissue culture friendly. In this study, tissue culture friendly cultivar Golden Promise (GP) and tissue culture resistant DWRB91(D91) were selected as contrasting cultivars to investigate the molecular basis of regeneration efficiency. Multiomics studies involving transcriptomics, proteomics, metabolomics, and biochemical analysis were performed using GP and D91 callus to unravel the regulatory mechanisms. Transcriptomics analysis revealed 1487 differentially expressed genes (DEGs), in which 795 DEGs were upregulated and 692 DEGs were downregulated in the GP-D91 transcriptome. Genes encoding proteins localized in chloroplast and involved in ROS generation were upregulated in the embryogenic calli of GP. Moreover, proteome analysis by LC-MSMS revealed 3062 protein groups and 16989 peptide groups, out of these 1586 protein groups were differentially expressed proteins (DEPs). Eventually, GC-MS based metabolomics analysis also revealed the higher activity of plastids and alterations in key metabolic processes such as sugar metabolism, fatty acid biosynthesis, and secondary metabolism. Higher accumulation of sugars, amino acids and metabolites corresponding to lignin biosynthesis were observed in GP as compared to D91. Highlights:Multi omics analysis revealed chloroplast play crucial role in providing in vitro regeneration capability in contrasting genotypes
Chaudhary Chanderkant、Baliyan Suchi、Poonia Anuj Kumar、Chauhan Harsh、Waghmode Bhairavnath、Sirohi Parul、Vishnoi Ritika、Sircar Debabrata、Mishra Sumit Kumar、Chaudhary Reeku、Germain Hugo
Department of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Biosciences and Bioengineering, Indian Institute of Technology RoorkeeDepartment of Chemistry, Biochemistry and Physics, University of Quebec Trois Rivieres
农业科学技术发展植物学生物科学研究方法、生物科学研究技术
In vitro regenerationchloroplastgenetic transformationtranscriptomicsproteomicsmetabolomics
Chaudhary Chanderkant,Baliyan Suchi,Poonia Anuj Kumar,Chauhan Harsh,Waghmode Bhairavnath,Sirohi Parul,Vishnoi Ritika,Sircar Debabrata,Mishra Sumit Kumar,Chaudhary Reeku,Germain Hugo.Chloroplast activity provides in vitro regeneration capability in contrasting cultivars[EB/OL].(2025-03-28)[2025-05-01].https://www.biorxiv.org/content/10.1101/2022.06.30.498295.点此复制
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