家蝇幼虫血淋巴抗菌组分的分离纯化与抗菌活性分析

Hemolymph extracted from Musca domestica larvae was treated with the mixtrue of living Escherichia coli and Staphylococcus aureus。 After acidification with trifluoroacetic acide, the hemolymph was fractionated on Sep-Pak C18 column with 10%, 30% and 80% of acetonitrile in succession and the crude components were concentrated by vacuum centrifuge, and then their antibacteiral activities were monitored with the method of cylinder-plate。 Further purification was conducted on crude components seperated with 10% and 30% of ACN through RP-HPLC, and antimicrobial activities of sub-components were measured with liquid growth inhibition method。 The results show that three and two sub-components in the crude component from 10% ACN of extract had high activity against Escherichia coli and Staphylococcus aureus respectively, and three sub-components from 30% of extract had distinguishable activity against Escherichia coli.