EGCG-CS-PAA纳米粒制备及其稳定性实验研究
Preservation of EGCG antioxidant properties by loading CS-PAA nanoparticles
为保护EGCG的抗氧化活性，利用壳聚糖（CS）和聚天冬氨酸（PAA）之间离子交联作用制备CS-PAA纳米粒载体，装载表没食子儿茶素没食子酸酯（EGCG）。结果表明：CS/PAA质量比、溶液pH值、搅拌时间以及盐浓度对纳米体系的粒径、Zeta电位 、包埋率等具有显著影响。在CS/PAA（w/w）为1.0、pH为3.5、反应时间为60min时，制备的EGCG-CS-PAA纳米粒在粒径和表面电荷等物理特征为最佳。采用FRAP法对EGCG-CS-PAA纳米粒的抗氧化活性进行分析，结果显示纳米体系对EGCG活性具有良好的保护作用。同时EGCG-CS-PAA纳米体系 能在高温、碱性等环境下起到较好的保护EGCG的作用。
(-)-Epigallocatechin-3-gallate (EGCG) was loaded in chitosan nanoparticles for the preservation of antioxidant activity. The effects of the weight ratio of CS to PAA, pH, the reaction time and the concentration of NaCl on the properties of EGCG-CS-PAA complexes were studied. All four factors significantly influenced the particle size, the ζ-potential, and the entrapment efficiency of EGCG. A stable and clear solution system could be obtained at pH 3.5. The best EGCG-CS-PAA nanoparticles was obtained with the reaction time at 1 hour and the weight ratio of 1:1 ( CS: PAA)．Sustained free radical (ABTSo＋) scavenging assays showed that the antioxidant activity of EGCG was retained by the nanoparticles. Meanwhile, EGCG nanoparticles were found to be a better protection of EGCG at high temperature and alkaline.
洪志勇、应浩、侯善欣、杜琪珍、黄美蓉、侯绍云
药学生物科学研究方法、生物科学研究技术
食品营养EGCG壳聚糖纳米粒抗氧化活性?????
Food nutritionEGCGchitosannanoparticlesantioxidant activity
洪志勇,应浩,侯善欣,杜琪珍,黄美蓉,侯绍云.EGCG-CS-PAA纳米粒制备及其稳定性实验研究[EB/OL].(2015-07-06)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201507-37.点此复制
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