3-羟基丁酸对体外培养的神经干细胞增殖及分化的影响
he effects of 3-hydroxybutyrate on the proliferation and differentiation of neural stem cells in vitro
目的：研究体外条件下3-羟基丁酸（3-HB）对神经干细胞（NSCs）增殖及分化的影响。方法：分离孕14.5天大鼠胚胎大脑皮质NSCs，进行原代培养；培养至第三代的NSCs分别用0.1、0.05、0.02、0.01g/l 3-HB处理，未加药组作为对照，CCK-8试剂盒检测细胞增殖、活力，免疫细胞荧光化学观察NSCs分化情况。结果：与对照组相比，3-HB处理对NSCs的增殖、活力没有显著影响；3-HB处理组β-tubulin阳性细胞（神经元）比例增加，GFAP阳性细胞（胶质细胞）比例没有显著变化。结论：3-HB对分离培养的NSCs在体外的增殖和活力无明显影响，但能够促进其向神经元方向分化。
Objective: Learn the effect of 3-hydroxybutyrate(3-HB) on the viability and differentiation of neural stem cell in vitro. Method: NSCs were isolated from the cerebral cortex of rat embryos on embryonic day 14-15, and P3 cells were used; NSCs were treated with different concentration(0.1、0.05、0.02、0.01g/l) of 3-HB, untreated as control. Cell viability was detected by CCK-8, and immune cell fluorescence chemistry was used to study the differentiation of NSCs. Result: Cell viability had no significant difference between 3-HB treated group and control. The percentage of β-tubulin positive cells was increased, but the percentage of GFAP positive cells had no dramatically alteration. Conclusion: Different concentration of 3-HB had no significant influence on NSCs viability, but could promote NSCs differentiate into neurons.
卢晓云、吕海侠、王媛媛、冯敏娟、李明川、焦倩、杨志倩
基础医学神经病学、精神病学细胞生物学
3-羟基丁酸神经干细胞细胞增殖细胞分化
3-HBneural stem cellcell proliferationcell differentiation
卢晓云,吕海侠,王媛媛,冯敏娟,李明川,焦倩,杨志倩.3-羟基丁酸对体外培养的神经干细胞增殖及分化的影响[EB/OL].(2012-02-10)[2025-05-11].http://www.paper.edu.cn/releasepaper/content/201202-235.点此复制
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