土壤中辛硫磷残留量的HPLC测定方法研究

Research of phoxim residue determination in soil by HPLC was conducted by optimizing Purification conditions, extraction conditions and Chromatographic conditions. The results showed that residue of phoxim were extracted from samples with Ethyl acetate (100L), bathed by a mixed column of mineral ether and Ethyl acetate (1/9, V/V), cleaning-up was performed on a column of neutral alumina. The target compound in samples were determined by HPLC with diode array detector at wavelength 280nm when mobile phase was acetonitrile with water, chromatographic column was ZORBAX? SB-C18 and current speed was 0.45mLomin-1, Target peak was got with balanced Base line, symmetrical peaking and fully separated from another impurity; The linear range of phoxim was 0.5-10μgomL-1 and regression equation was y=19.88392x+5.50191e-1(r=0.99998), and recoveries of spiked samples reached 87.0%. This analysis method with characters of simple, speediness, nicety and delicacy was fit for the detecting of phoxim residue determination in soil.