重庆市禽类鹦鹉热衣原体流行情况调查
Poultry epidemic survey of avian Chlamydia psittace in Chongqing
对重庆市6个区县不同养殖环境下的禽类随机采集血清样品400份和相对应的组织样品430份，利用IHA诊断试剂盒初步检测筛选出可疑样品，然后采用标准鸟源鹦鹉热衣原体引物通过PCR反应对可疑样品进行进一步检测，以评价重庆市禽类鹦鹉热衣原体的流行情况。结果表明，IHA检测的阳性率为39.3%，PCR复检阳性样品的阳性率为36.5%；6个区县的5种禽类中均检出了衣原体阳性，各个地区和种类间的感染率介于26.0%-45.0%。说明重庆市地区的禽类均有较大比例的鹦鹉热衣原体感染。通过对IHA检测的阳性样品进行PCR检测表明，PCR检测方法的准确性更高，所建立的鹦鹉热衣原体PCR检测体系可用于禽类鹦鹉热衣原体检测。
In order to evaluate the prevalence of Chlamydia psittaci in Chongqing，we had stochastically collected 400 blood samples and 430 tissue samples of poultry in different feeding conditions from six districts of Chongqing. These samples were detected firstly by IHA for Chalmydia psittaci, these suspected samples were detected again by PCR using standard Chalmydia psittaci primers. The results showed that the positive rates of Chlamydia psittaci were 39.0% (IHA) and 36.5% (PCR) respectively. The high positive rates (26.0%-45.0%) were found in the samples from all of the 6 districts and 5 kinds of poultry from Chongqing. It indicated that the poultry is widely infected by Chlamydia psittaci in Chongqing. The PCR method can be used in the Chlamydia psittaci detection, and it’s more exact than IHA.
邓伟、杨迎伍、李应国、王国民、李正国、吴东海
微生物学医学研究方法预防医学
重庆禽类鹦鹉热衣原体调查
hongqingpoultryChalmydia psittacisurvey
邓伟,杨迎伍,李应国,王国民,李正国,吴东海.重庆市禽类鹦鹉热衣原体流行情况调查[EB/OL].(2007-07-03)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/200707-42.点此复制
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