加味四物汤对荷骨肉瘤小鼠肿瘤VEGF及其受体表达的影响

Objective To investigate the effect of modified Decoction of Four Drugs on VEGF ，Flt-1 and　KDR expression in the osteosarcoma mouse. Methods　We build the mice orthotropic transplantation model of osteogenic sarcoma KHOS. After treating with the modified Decoction of Four Drugs by intragastric administration; measure the expression of VEGF, KDR, Flt-1 of the transplantation tumor by the way of RT-PCR method.　Results　 The high-dose modified Decoction of Four Drugs group has the effect of significantly inhibiting the expression of VEGF,KDR and Flt-1 of the transplantation tumor tissue, while the effect of inhibiting the expression of VEGF,KDR and Flt-1 of the transplantation tumor tissue by the middle and low-dose groups is not significant; the effect of the high-dose Chinese medicine group on inhibiting the expression of VEGF,KDR and Flt-1 of the transplantation tumor tissue is better than that of the middle and low-dose groups(P<0.05).Conclusion The invasion of bone tumor can be depressed by reducing the expression of VEGF,KDR and Flt-1 to control the growth of new vessels of tumor in order to interrupt the in vivo growth and inhibition of malignant bone tumor. but it relates to density.