微生物的分子生物学鉴定方法

he classification and identification of the microorganisms is an important way to the study of microorganism. Traditionally, it is tedious and time-consuming because it follows the character of bioecology .With the development of molecular biology, the way of microbic identification becomes more precise. Most of the molecular biology methods are involved in PCR, for instants, temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis, random sequencing in clone libraries, single-strand conformation polymorphism, terminal-restriction fragment length polymorphism, and so on. Some methods which are not involved in PCR are also be used widely, such as fluorescence in-situ hybridization, DNA re-association analysis. On the whole, we can get different information from different methods. In this paper, we introduce some useful methods and show their advantages and disadvantages.