ZNF692 organizes a hub for ribosome maturation enhancing translation in rapidly proliferating cells
ZNF692 organizes a hub for ribosome maturation enhancing translation in rapidly proliferating cells
Abstract Rapidly proliferating cells produce more ribosomes to translate sufficient proteins for cell growth. One of the first and rate limiting steps in translation initiation is the interaction of the small ribosomal subunit with mRNAs. Therefore, effective small ribosomal subunit biogenesis is critical for translation initiation efficiency. Here we report the identification of the zinc finger protein 692 (ZNF692), a MYC-induced nucleolar scaffold that coordinates the final steps in the biogenesis of the small 40S ribosome. ZNF692 forms a complex with rRNA, the 90S processome and the nucleolar exosome in the granular component of the nucleolus creating a hub specialized in the final steps of 18S processing and small ribosomal subunit maturation. Cancer cells are more reliant on ZNF692 for increased translation than normal cells. We propose that MYC increases translation efficiency by promoting the expression of ZNF692, adjusting the translation rate to the increase in mRNA transcription induced by MYC.
Brown Isabella N.、Tran Vanna M.、Lafita-Navarro M.Carmen、Hao Yi-Heng、Venkateswaran Niranjan、Han Jungsoo、Mettlen Marcel、Conacci-Sorrell Maralice、Jang Seoyeon、Grishin Nick V、Kinch Lisa、Jiang Chunhui、Stachera Weronika、Mendell Joshua T.、Mundy Dorothy、Buszczak Michael、Woodruff Jeffrey B.、Chang Tsung-Cheng、Maurais Elizabeth
Department of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Molecular Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical Center||Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center||Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical CenterDepartment of Molecular Biology, University of Texas Southwestern Medical CenterHoward Hughes Medical Institute, University of Texas Southwestern Medical Center||Department of Biophysics, University of Texas Southwestern Medical Center Dallas TX USAHoward Hughes Medical Institute, University of Texas Southwestern Medical Center||Department of Biophysics, University of Texas Southwestern Medical Center Dallas TX USADepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Molecular Biology, University of Texas Southwestern Medical Center||Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center||Howard Hughes Medical Institute, University of Texas Southwestern Medical Center||Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical Center||Live Cell Imaging Core Facility, University of Texas Southwestern Medical CenterDepartment of Molecular Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical CenterDepartment of Molecular Biology, University of Texas Southwestern Medical CenterDepartment of Cell Biology, University of Texas Southwestern Medical Center
基础医学分子生物学细胞生物学
MYCnucleolusribosome biogenesisprotein synthesisZNF692ZFP692NPM1rRNAexosome18SEXOSC7EXOSC8KRR140S
Brown Isabella N.,Tran Vanna M.,Lafita-Navarro M.Carmen,Hao Yi-Heng,Venkateswaran Niranjan,Han Jungsoo,Mettlen Marcel,Conacci-Sorrell Maralice,Jang Seoyeon,Grishin Nick V,Kinch Lisa,Jiang Chunhui,Stachera Weronika,Mendell Joshua T.,Mundy Dorothy,Buszczak Michael,Woodruff Jeffrey B.,Chang Tsung-Cheng,Maurais Elizabeth.ZNF692 organizes a hub for ribosome maturation enhancing translation in rapidly proliferating cells[EB/OL].(2025-03-28)[2025-05-28].https://www.biorxiv.org/content/10.1101/2022.05.26.493655.点此复制
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