Silencing of ANKRD12 circRNA induces molecular and functional changes associated with invasive phenotypes
Silencing of ANKRD12 circRNA induces molecular and functional changes associated with invasive phenotypes
ABSTRACT Circular RNAs (circRNA) that form through non-canonical backsplicing events of pre-mRNA transcripts are evolutionarily conserved and abundantly expressed across species. However, the functional relevance of circRNAs remains a topic of debate. In this study, we identified and characterized a circular RNA derived from Exon 2 and Exon 8 of the ANKRD12 gene, termed here as circANKRD12. We show that this circRNA is abundantly expressed in breast and ovarian cancers. The circANKRD12 is RNase R resistant and predominantly localized in the cytoplasm in contrast to its source gene mRNA. We confirmed the expression of this circRNA across a variety of cancer cell lines and provide evidence for its functional relevance through downstream regulation of several tumor invasion genes. We show that silencing of circANKRD12 induces a phenotypic change by significantly regulating cell cycle, increasing invasion and migration, altering the metabolism in cancer cells. These results reveal the functional significance of circANKRD12 and provide evidence of a regulatory role for this circRNA in cancer progression.
Mohamoud Yasmin Ali、Malek Joel A.、Rafii Arash、Al-Azwani Iman K.、Karedath Thasni、Ameri Wafa Al、Andrews Simeon S.、Samuel Samson、Ahmed Ikhlak、Al-Dasim Fatima M.
Genomics Core, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Genetic medicine, Weill Cornell Medicine-Qatar, Education City, Qatar Foundation||Genomics Core, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationStem Cell and Microenvironment Laboratory, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationGenomics Core, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Genetic medicine, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Genetic medicine, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Genetic medicine, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Pharmacology, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Genetic medicine, Weill Cornell Medicine-Qatar, Education City, Qatar FoundationDepartment of Genetic medicine, Weill Cornell Medicine-Qatar, Education City, Qatar Foundation
肿瘤学分子生物学基础医学
Circular RNARNA-seqBreast cancersiRNACancer InvasionOXPHOS
Mohamoud Yasmin Ali,Malek Joel A.,Rafii Arash,Al-Azwani Iman K.,Karedath Thasni,Ameri Wafa Al,Andrews Simeon S.,Samuel Samson,Ahmed Ikhlak,Al-Dasim Fatima M..Silencing of ANKRD12 circRNA induces molecular and functional changes associated with invasive phenotypes[EB/OL].(2025-03-28)[2025-05-13].https://www.biorxiv.org/content/10.1101/366245.点此复制
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