蓝光对体外培养视网膜色素上皮细胞分泌VEGF及PEDF的影响

Objective To investigate the effect of blue light exposure on vascular endothelial growth factor (VEGF) and pigment epithelium derived factor (PEDF) secretion of cultured retinal pigment epithelium (RPE) cells laden with the lipofuscin fluorophore N-retinylidene-N-retinylethanolamine (A2E). Methods The A2E -laden RPE cells were exposed to blue light (450±9 nm) light with A2E (30 μmol/l).The light intensity was 50mW/mm2.According to blue light exposure time, RPE cells were divided into: group A (10 min) ; group B (30 min) ; group C( 60 min) ; group D (without light). The viability of the cells was analysed, the level of VEGF and PEDF were determined by enzyme - linked immunosorbent assay. Results Cell viability of A, B, C and D group was( 70.11±4.96)% 、( 49.87±6.83)%、( 31.29±5.78)%、( 90.28±1.9)% respectively; VEGF/PEDF ratio was 1.14、10.88、19.42、0.88 respectively. Conclusions Blue light could induce VEDF/PEDF imbalance of A2E-containing RPE cells.