基于玻璃电泳芯片分离STR片段的研究
Electrophoretic Analysis of Short Tandem Repeats (STR) in glass chip
在普通玻璃材料的电泳芯片上，利用激光诱导荧光检测系统实现了对单链STR（短串联重复序列）片段快速高效的分离检测和优化研究。选用分离通道为8.5cm玻璃芯片，用ABI公司生产的POP-4和POP-7筛分胶来分离法医鉴定试剂盒扩增的DNA片段。通过改变酸碱清洗芯片的顺序研究清洗芯片对于分离STR片段的影响，通过改变电压及筛分胶的配比来优化筛分效果，提高分离度。实验结果表明，改变酸碱清洗顺序影响出峰时间和分离度，将POP-4和POP-7混合使用比单独使用效果要好，改变电压对分离度也有一定影响。
he aim of the study is to investigate the factors that affect separation efficiency of STR single-stranded DNA fragments on glass chip-capillary electrophoresis (chip-CE), detected by single-colour laser-induced fluorescence detection system. The STR fragments were amplified by AmpFlSTR? Identifiler? Plus Kit (ABI) in a common PCR thermal cycler, and the fragments were analyzed through POP-4 and POP-7 gel-matrix mixture (ABI) in a glass channel (8.5 cm long) on a glass chip. The experiments showed that the order of glass channel acid and alkali cleaning procedure has greater impact on the retention time and resolution; mixture of POP-4 and POP-7 obtained better resolution than that of using POP-4 or POP-7 alone; and separation voltage also influences the separation resolution.
何越、荣吉赞、艾洪新、杨楠
生物科学研究方法、生物科学研究技术生物工程学遗传学
电泳芯片激光诱导荧光检测系统STR片段酸碱清洗POP-4和POP-7
chip-CElaser-induced fluorescence detection systemSTR fragmentsAcid and alkali cleaningPOP4 and POP-7
何越,荣吉赞,艾洪新,杨楠.基于玻璃电泳芯片分离STR片段的研究[EB/OL].(2013-04-24)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201304-465.点此复制
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