拟南芥rd29A启动子的克隆及其在甘蔗抗逆转基因中的应用初探

he 420 bp region of rd29A gene promoter from Arabidopsis thaliana genome was amplified by PCR technique. Sequence analysis showed that the fragment contained two cis-acting elements including dehydration responsive elements (DRE) and ABA responsive elements (ABRE) and the main regulation motifs. The plant expression vector pPrd-S65T was constructed with this fragment linked up with green fluorescent protein (GFP) gene controlled by rd29A promoter, which was transferred to sugarcane callus by bombardment microprojectiles. The expressions of gfp in the transformed sugarcane callus treated with 250 mmol/L NaCl for3 days and 5 days, were detected by fluorescent microscope.The result indicated that the cloned rd29A promoter could be applied in the transgenic sugarcane for the stress resistance.