Molecular basis of differential adventitious rooting competence in poplar genotypes
Molecular basis of differential adventitious rooting competence in poplar genotypes
Abstract Recalcitrant adventitious root (AR) development is a major hurdle in propagating commercially important woody plants. Although significant progress has been made to identify genes involved in subsequent steps of AR development, the molecular basis of differences in apparent recalcitrance to form AR between easy-to-root and difficult-to-root genotypes remains unknown.To address this, we generated cambium tissue-specific transcriptomic data from stem cuttings of hybrid aspen, T89 (difficult-to-root) and hybrid poplar OP42 (easy-to-root) and used transgenic approaches to verify the role of several transcription factors (TF) in the control of adventitious rooting.Increased peroxidase activity is positively correlated with better rooting. We found differentially expressed genes encoding Reactive Oxygen Species (ROS) scavenging proteins to be enriched in OP42 compared to T89. A higher number of differentially expressed TF in OP42 compared to T89 cambium cells was revealed by a more intense transcriptional reprograming in the former. PtMYC2, a potential negative regulator, was less expressed in OP42 compared to T89. Using transgenic approaches, we have demonstrated that PttARF17.1 and PttMYC2.1 negatively regulate adventitious rooting.Our results provide insights into the molecular basis of genotypic differences in AR and implicate differential expression of the master regulator MYC2 as a critical player in this process.
Ranjan Alok、Brunoni Federica、Alallaq Sanaria、Singh Rajesh、Perrone Irene、Chitarra Walter、Martin Francis、Guinet Frederic、Street Nathaniel、Bellini Catherine、Rigal Adeline、Legu¨| Val¨|rie、Kohler Annegret、Bhalerao Rishikesh
Ume? Plant Science Centre, Department of Plant Physiology, Ume? UniversityUme? Plant Science Centre, Department of Plant Physiology, Ume? UniversityUme? Plant Science Centre, Department of Plant Physiology, Ume? University||Department of Biology, College of Science for Women, Baghdad UniversityUme? Plant Science Centre, Department of Forest Genetics and Physiology, Swedish Agriculture UniversityUme? Plant Science Centre, Department of Plant Physiology, Ume? University||Institute for Sustainable Plant Protection, National Research Council of Italy (IPSP-CNR)Institute for Sustainable Plant Protection, National Research Council of Italy (IPSP-CNR)||Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE)Universit¨| de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Laboratory of Excellence ARBRE, INRAE GrandEst-NancyUniversit¨| de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Laboratory of Excellence ARBRE, INRAE GrandEst-NancyUme? Plant Science Centre, Department of Plant Physiology, Ume? UniversityUme? Plant Science Centre, Department of Plant Physiology, Ume? University||Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Universit¨| Paris-SaclayUniversit¨| de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Laboratory of Excellence ARBRE, INRAE GrandEst-NancyUniversit¨| de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Laboratory of Excellence ARBRE, INRAE GrandEst-NancyUniversit¨| de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Laboratory of Excellence ARBRE, INRAE GrandEst-NancyUme? Plant Science Centre, Department of Forest Genetics and Physiology, Swedish Agriculture University
分子生物学植物学遗传学
adventitious rootscambiumhybrid aspenhybrid poplarPopulus spp.stem cuttings
Ranjan Alok,Brunoni Federica,Alallaq Sanaria,Singh Rajesh,Perrone Irene,Chitarra Walter,Martin Francis,Guinet Frederic,Street Nathaniel,Bellini Catherine,Rigal Adeline,Legu¨| Val¨|rie,Kohler Annegret,Bhalerao Rishikesh.Molecular basis of differential adventitious rooting competence in poplar genotypes[EB/OL].(2025-03-28)[2025-05-23].https://www.biorxiv.org/content/10.1101/2021.09.14.460203.点此复制
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