猕猴桃种质遗传多样性的EST-SSR分析

Eleven pairs of EST-SSR markers developed and designed by our lab were used to amplify the genomic DNA isolated from 33 kiwifruit genotypes (Actinidia spp.) in order to analyze their genetic diversity and relationship. The results showed that the clear DNA fragments were amplified for all materials tested by using 11 pair of EST-SSR primers, among which 8 of them were showed polymorphic, accounting for 72.7%, the identification rates of 33 germplasms were up to 100 %. Totally 61 alleles were detected by the 8 polymorphic pair of EST-SSR primers, ranging from 2 to 17 (mean 7.6) for each polymorphic primer set. Then Cluster analysis results by NTSYS-pc software based on Unweighted pair-group method arithmetic average (UPGMA) showed that the Dice similarity coefficients of 33 germplasms of kiwifruit ranged from 0.60 to 0.97, it suggested that their genetic relationships were relatively near. Thirty three accessions could be divided into seven groups according to the Dice similarity coefficient 0.73, which was consistent with the traditional classification in certain degree. It could reflect the kiwifruit genetic variation on molecular level by EST-SSR markers and provide theoretical basis for kiwifruit breeding. It is no doubt that EST-SSR markers are very effective to evaluate DNA polymorphism and genetic diversity in kiwifruit germplasms, and will provide theoretical information for molecular breeding and genetic mapping in kiwifruit.