小鼠乳腺中FGF7及其受体的定位与表达

o invest the dynamic changes in expression and localization of fibroblast growth factor 7（FGF7）and its receptor in mammals development, lactation and involution, immunofluorescence assay was used to detect FGF7 by a laser scanning confocal microscope firstly in mammary gland slices. The results revealed that: FGF7 mainly localized in the peripheral of mammary gland ductal epithelial cells and acinar cells , and a weak expression in matrix .The expression of FGF7 had a few notable high points: virgin 20d, virgin 40d, the early and middle of pregnancy, early lactation, and the initial and the late involution. The expression of FGF7 increased to the highest value in involution 4d among these periods.KGFR occurred mainly in the ductal epithelial cells and acinar cells, and a weak expression in matrix in the individual time. The higher expression of KGFR in virgin was 20d, 60d; In pregnancy, the KGFR level cutted down slightly first, and increased significantly after pregnancy 10d, then declined slightly after up to pregnancy 16d. In lactation, the expression of KGFR was highest in 4d and then fallen untill involution. The expression of KGFR increased significantly from involution 19d.